讲座主题：Robotic Enucleation for Oocytes and Cloned pigs produced by Robotic SCNT
讲座时间：2018年1月29日 9：00-11:30
讲座地点：机械楼 A810
报 告 人：Xin Zhao
Institute of Robotics and Automatic Information System, Nankai University, Tianjin 300071, China.
【Abstract 】
The micro-manipulation (MM) technique is crucial approaches to operate cells. The traditional MM methods ignore the mechanical properties of cells, which make them have lower operation accuracy and easily cause damage of the cells. Somatic cell nuclear transfer ( SCNT ) is one of the most complex cell manipulations, the major challenge in robotic SCNT is to increase the development rate of reconstructed embryos. Robotic SCNT includes three steps: oocyte rotation to find polar body, oocyte enuleation, and somatic cell injection. We analyzed mechanical properties of oocytes, and proposed the oocyte rotation and oocyte enuleation methods based on minimum forces. Experiment results show that the proposed robotic SCNT system reduce the mechanical damage of the oocytes, and lead to high development rate. Furthermore, robotic SCNT has been applied to pig cloning. We did thousands of robotic SCNT operations and transferred 510 reconstructed embryos to 6 pigs, and obtained 13 cloned pigs at last. Our results demonstrate that the robotic SCNT not only relieves the operator from tedious cell operations, but also reduces the damage of the oocytes in SCNT.
【Biography】
Xin Zhao received the B.S. degree from Nankai University, Tianjin, P.R.China, in 1991, the M.S. degree from Shenyang Institute of Automation, CAS, Shenyang, P.R.China, in 1994 , and the Ph.D. degree from Nankai University, in 1997, all in control theory and control engineering.
He joined the faculty at Nankai University, Tianjin, P.R.China in 1997, he is Professor and Vice Dean of College of Computer and Control Engineering. His research interests are in Mico-Nano Manipulation and System, including Micro manipulator, Micro system and Mathematical Biology.
Prof. Zhao was the recipient of 1999 Excellent Professor Award, Nankai University, 2000 Inventory Prize, Tianjin Municipal Government, 2002 Excellent Professor Award of “College Key Teachers Fund”, Ministry of Education, 2002 Excellent Professor Award of “Baogang Fund” and 2007 Program for New Century Excellent Talents in University, Ministry of Education. His team pioneered robotic animal cloning and successfully got 13 cloned piglets in 2017.
